Display of a β-mannanase and a chitosanase on the cell surface of Lactobacillus plantarum towards the development of whole-cell biocatalysts

Table 2 Primers used in this study

Primer	Sequence^a 5′→3′	Restriction site underlined
Man3050F	TGCTTCATCAGTCGAC CACACCGTTTCTCCGGT	SalI
ManR1	TGAGATGAGTTTTTGTTCTTCCACGACAGGCGTCAAAGAAT
ManR2	TCAGATCCTCTTCTGAGATGAGTTTTTGTTCTTCCACGAC
3050R3	GTTCAGTGACACGCG T CAGATCCTCTTCTGAGATG	MluI
Man1261F	GATTGCGGCGGTCGAC CACACCGTTTCTCCGGT	SalI
1261R3	CCGGGGTACCGAATTC TTACAGATCCTCTTCTGAGATG	EcoRI
Csn3050F	TGCTTCATCAGTCGAC GCGGGACTGAATAAAGATC	SalI
CsnR1	TGAGATGAGTTTTTGTTCGTCGACAGATCCTTTGATTAC
CsnR2	CAGATCCTCTTCTGAGATGAGTTTTTGTTCGTCGACAGA
Csn1261F	GATTGCGGCGGTCGAC GCGGGACTGAATAAAGATC	SalI

^aThe nucleotides in italics are the positions that anneal to the DNA of the target gene (manB or cnsA)

ISSN: 1475-2859