Table 1 Strains and plasmids used in this study

Strains

 L. plantarum WCFS1

Host strain

[8]

 E. coli TOP10

Host strain

Invitrogen

Plasmids

 pEV

Erm^r; pLp_2578sAmyA derivative, no signal sequence, no man or csn (negative control)

[16]

 pSIP409-ManB-native

Erm^r; spp- based expression vector pSIP409, for expression of manB its native signal peptide

[28]

 pSIP409-CsnA-native

Erm^r; spp- based expression vector pSIP409 for expression of csnA with native signal peptide

[28]

 pLp_1261InvS

Erm^r; pSIP401 derivative with the lipoanchor sequence from Lp_1261 fused to part of the inv gene.)

[16]

 pLp_3050_DC_Ag85B_E6_cwa2

Erm^r; pSIP401 derivative encoding the Lp_3050 signal peptide ([32]) translationally fused to the hybrid antigen DC-Ag85B-ESAT6, followed by the cell wall anchor (cwa2) from Lp_2578 (cwa2 comprises 194 residues of Lp_2578; [17])

(Unpublished)

 pSIP_1261ManB

Erm^r; pLp_1261InvS derivative with manB-myc instead of the inv gene

This study

 pSIP_1261CsnA

Erm^r; pLp_1261InvS derivative with csnA-myc instead of the inv gene

This study

 pSIP_ManBcwa2

Erm^r; pLp_3050_DC_Ag85B_E6_cwa2 derivative with manB-myc instead of the gene fragment encoding DC_Ag85B-E6

This study

 pSIP_CsnAcwa2

Erm^r; pLp_3050_DC_Ag85B_E6_cwa2 derivative with csnA-myc instead of the gene fragment encoding DC_Ag85B-E6

This study