Skip to main content

Table 1 Strains and plasmids used in this study

From: Display of a β-mannanase and a chitosanase on the cell surface of Lactobacillus plantarum towards the development of whole-cell biocatalysts

Strain or plasmid Relevant characteristic (s) Reference source
Strains
 L. plantarum WCFS1 Host strain [8]
 E. coli TOP10 Host strain Invitrogen
Plasmids
 pEV Ermr; pLp_2578sAmyA derivative, no signal sequence, no man or csn (negative control) [16]
 pSIP409-ManB-native Ermr; spp- based expression vector pSIP409, for expression of manB its native signal peptide [28]
 pSIP409-CsnA-native Ermr; spp- based expression vector pSIP409 for expression of csnA with native signal peptide [28]
 pLp_1261InvS Ermr; pSIP401 derivative with the lipoanchor sequence from Lp_1261 fused to part of the inv gene.) [16]
 pLp_3050_DC_Ag85B_E6_cwa2 Ermr; pSIP401 derivative encoding the Lp_3050 signal peptide ([32]) translationally fused to the hybrid antigen DC-Ag85B-ESAT6, followed by the cell wall anchor (cwa2) from Lp_2578 (cwa2 comprises 194 residues of Lp_2578; [17]) (Unpublished)
 pSIP_1261ManB Ermr; pLp_1261InvS derivative with manB-myc instead of the inv gene This study
 pSIP_1261CsnA Ermr; pLp_1261InvS derivative with csnA-myc instead of the inv gene This study
 pSIP_ManBcwa2 Ermr; pLp_3050_DC_Ag85B_E6_cwa2 derivative with manB-myc instead of the gene fragment encoding DC_Ag85B-E6 This study
 pSIP_CsnAcwa2 Ermr; pLp_3050_DC_Ag85B_E6_cwa2 derivative with csnA-myc instead of the gene fragment encoding DC_Ag85B-E6 This study