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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Display of a β-mannanase and a chitosanase on the cell surface of Lactobacillus plantarum towards the development of whole-cell biocatalysts

Fig. 1

Schematic overview of the expression cassette for N-terminal lipoprotein anchoring (a) and C-terminal cell wall anchoring (b) of mannanase (ManB) and chitosanase (CsnA) in L. plantarum. The vectors are derivatives of previously described plasmids [16, 17, 31]. The inserts containing the manB or csnA sequence fused with a Myc tag for protein detection. All parts are easily interchangeable using the indicated linker (L) restriction sites (SalI and EcoRI or MluI). See text for more details

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