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Fig. 9 | Microbial Cell Factories

Fig. 9

From: Efficient evaluation of cellulose digestibility by Trichoderma reesei Rut-C30 cultures in online monitored shake flasks

Fig. 9

Characteristic growth and enzyme production parameters of T. reesei Rut-C30 on OrganoCat and OrganoSolv cellulose. T. reesei Rut-C30 was grown on 5 g L−1 glucose and 30 g L−1 α-cellulose, 33 g L−1 OrganoCat-cellulose, 33 g L−1 OrganoSolv-cellulose, supplemented with 0.3 g L−1 urea, 0.1 % (v/v) tween 80 and 2 g L−1 peptone. a Oxygen transfer rate (OTR) and protein content in the culture supernatant; b pH and filter paper activity. The OTR was measured in duplicates. As the maximum mean error was as low as 1 mmol L−1 h−1, average values are shown. For clarity only every second measuring point over time is represented by a symbol. Error bars represent standard deviation of technical triplicates. Culture conditions: modified Pakula medium, 250 mL flask, filling volume 20 mL, shaking frequency 350 rpm, shaking diameter 50 mm, inoculum 106 spores mL−1, and 30 °C

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