Fig. 6
Addition of fresh cellulose at different time points to the culture of T. reesei Rut-C30. T. reesei Rut-C30 was grown on 5 g L−1 glucose and 30 g L−1 cellulose. a Duplicates of oxygen transfer rate (OTR) and c cumulative oxygen transfer (OT) during growth on α-cellulose. 10 g L−1 α-cellulose was added after 51 and 72 h of cultivation to different shake flasks; b duplicates of oxygen transfer rate (OTR) and d cumulative oxygen transfer (OT) during growth on Sigmacell 101. 10 g L−1 Sigmacell 101 was added after 45 and 62 h of cultivation to different shake flasks. The start of the time axes was set to 12 h to increase the visibility of the results. Prior to cellulose addition, all curves are depicted as black circles like the reference cultivation. Addition time points are marked by arrows. For clarity only every fourth measuring point over time is represented by a symbol. Culture conditions: modified Pakula medium, 250 mL flask, filling volume 20 mL, shaking frequency 350 rpm, shaking diameter 50 mm, inoculum 106 spores mL−1, and 30 °C