Fig. 5

Addition of cellulases at different time points to the culture of T. reesei Rut-C30. T. reesei Rut-C30 was grown on 5 g L⁻¹ glucose and 30 g L⁻¹ α-cellulose. Oxygen transfer rate (OTR) after addition of 6 IU mL⁻¹ of β-glucosidase, 6 FPU mL⁻¹ Celluclast or the combination of both enzymes at 32 h (a), 65 h (b) and 77 h (c). For comparison, cultures without any addition and cultures with addition of 1 mL 0.1 M sodium acetate buffer (pH 4.8), used as solvent for the enzymes, are shown. The start of the time axes was set to 12 h to increase the visibility of the results. Prior to enzyme addition, all curves are depicted as black circles like the reference cultivation. Addition time points are marked by arrows. For clarity only every second measuring point over time is represented by a symbol. Culture conditions: modified Pakula medium, 250 mL flask, filling volume 20 mL, shaking frequency 350 rpm, shaking diameter 50 mm, inoculum 10⁶ spores mL⁻¹, and 30 °C