Fig. 7
From: Functional inclusion bodies produced in the yeast Pichia pastoris
Assessment of cell penetration of VP1GFP IBs exposed to mammalian cells by 3D reconstruction of confocal images. VP1GFP IBs (green signals) were produced in a E. coli MC4100, (b) HY clone, and c LY clone of P. pastoris. Membrane (red) and nuclear (blue) labelling is apparent. In d, three-dimensional reconstruction of confocal microscopy images from VP1GFP IBs produced in either E.coli or P. pastoris. IBs were stained with cell mask membrane labelling (red). Side view signal is displayed next to each image. The scale bar represents 500Â nm