Fig. 4
From: Functional inclusion bodies produced in the yeast Pichia pastoris
Ultrastructural assessment of VP1GFP IBs in the yeast Pichia pastoris. General immuno-TEM view and detail of GFP labeling of entire yeast cells showing well defined intracellular IBs not surrounded by membrane (a), and isolated IBs (b). Note the heavy immunolocalization revealed by gold particles in both intracellular IBs and purified IBs, demonstrating the significant amounts of GFP in these nanoparticles. Dashed lines are included to stress the IB surfaces. c General FESEM view and detail of purified IBs showing their homogenous size, round shape, and rough surface. Bar size: a, c 0.5 μm; B 0.2 μm