Fig. 3
From: Functional inclusion bodies produced in the yeast Pichia pastoris
Characterization of VP1GFP produced in P. pastoris. a Anti-GFP blotting of VP1GFP soluble (S) and insoluble (I) cell fractions: M molecular weight standard (EZ-RUN Pre-Stained Rec Protein Ladder, reference BP3603). b Amount of insoluble VP1GFP vs soluble VP1GFP produced in both LY and HY clones. c Specific fluorescence of insoluble VP1GFP purified from P. pastoris compared with IBs produced in E. coli MC4100. (**p < 0.001). Specific fluorescence activity was estimated by the relation between the fluorescence units and the protein amount determined by Western blot