Table 4 Experimental design for 2D-DIGE analysis of proteins extracted from mycelium of different P. chrysogenum strains
Cy3 (50 µg of protein) | Cy5 (50 µg of protein) | Cy2 (50 µg total protein, 3.125 µg each sample) | |
|---|---|---|---|
Gel 1 | W1 | G1 | W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4 |
Gel 2 | Δ1 | W2 | W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4 |
Gel 3 | W3 | Δ*1 | W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4 |
Gel 4 | Δ2 | G2 | W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4 |
Gel 5 | Δ*2 | Δ3 | W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4 |
Gel 6 | G3 | Δ*3 | W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4 |
Gel 7 | Δ*4 | W4 | W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4 |
Gel 8 | G4 | Δ4 | W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4 |