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Table 4 Experimental design for 2D-DIGE analysis of proteins extracted from mycelium of different P. chrysogenum strains

From: Proteomic analysis of the signaling pathway mediated by the heterotrimeric Gα protein Pga1 of Penicillium chrysogenum

  Cy3 (50 µg of protein) Cy5 (50 µg of protein) Cy2 (50 µg total protein, 3.125 µg each sample)
Gel 1 W1 G1 W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4
Gel 2 Δ1 W2 W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4
Gel 3 W3 Δ*1 W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4
Gel 4 Δ2 G2 W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4
Gel 5 Δ*2 Δ3 W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4
Gel 6 G3 Δ*3 W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4
Gel 7 Δ*4 W4 W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4
Gel 8 G4 Δ4 W1 + W2 + W3 + W4 + Δ1 + Δ2 + Δ3 + Δ4 + Δ*1 + Δ*2 + Δ*3 + Δ*4 + G1 + G2 + G3 + G4
  1. Strains: W = Wis54-1255, G = PgaG42Rpyr-T, Δ = Δpga1, Δ* = Δpga1 + cAMP
  2. The number after the letter of the strain is the sample number; each sample comes from mycelium of an independent culture