Proteomic analysis of the signaling pathway mediated by the heterotrimeric Gα protein Pga1 of Penicillium chrysogenum

Table 3 Penicillium chrysogenum strains used for the proteomic analysis, their genotypes and Pga1 activities

Strain	Genotype/Pga1 function	Reference
Wis54-1255^a	Wild type pga1, normal Pga1 function	ATCC 28089
PgaG42Rpyr-T	pga1 ^G42R, constitutively active Pga1 subunit	García-Rico et al. [8, 10]
Δpga1	pga1 gene deleted, absent Pga1 subunit	García-Rico et al. [8, 10]
Δpga1 + cAMP^b	pga1 gene deleted, absent Pga1 subunit (intracellular cAMP levels increased)^b	García-Rico et al. [8, 10]

^aAlso known as Wisconsin 54-1255
^bdb-cAMP and theophylline were used to increase the level of intracellular cAMP

ISSN: 1475-2859