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Table 3 Penicillium chrysogenum strains used for the proteomic analysis, their genotypes and Pga1 activities

From: Proteomic analysis of the signaling pathway mediated by the heterotrimeric Gα protein Pga1 of Penicillium chrysogenum

Strain Genotype/Pga1 function Reference
Wis54-1255a Wild type pga1, normal Pga1 function ATCC 28089
PgaG42Rpyr-T pga1 G42R, constitutively active Pga1 subunit García-Rico et al. [8, 10]
Δpga1 pga1 gene deleted, absent Pga1 subunit García-Rico et al. [8, 10]
Δpga1 + cAMPb pga1 gene deleted, absent Pga1 subunit (intracellular cAMP levels increased)b García-Rico et al. [8, 10]
  1. aAlso known as Wisconsin 54-1255
  2. bdb-cAMP and theophylline were used to increase the level of intracellular cAMP