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Table 2 Prediction of the protein kinases targeting the phosphorylated sites identified by LC–MS/MS in protein Pc22g05690

From: Proteomic analysis of the signaling pathway mediated by the heterotrimeric Gα protein Pga1 of Penicillium chrysogenum

Site Kinase Score Phosphopeptides identified (LC–MS/MS)
T-279 PKA 0.64 (R)KRpTSIFGTLLGK(K)
S-280 PKA 0.64 (R)KRTpSIFGTLLGK(K)
(K)RTpSIFGTLLGK(K)
(R)TpSIFGTLLGK(K)
T-284 PKC 0.85 (R)KRTSIFGpTLLGK(K)
S-323 (K)AAEPTTESpSAEAPAPVAAETAETAAAPTK(A)
T-351 CKII 0.55 (K)AAEPTAEpTPAETTEAAKEEAK(D)
S-377 PKA 0.78 (K)RApSIFGNFFQK(V)
S-388 p38MAPK 0.56 (K)VApSPSQEK(S)
(K)VApSPSQEKSEK(E)
S-462 PKA 0.8 (K)RRTpSFFGNLGMK(K)
S-477 CKII 0.66 (K)EKKADpSDNEVTDGEAK(E)
(K)ADpSDNEVTDGEAK(E)
(K)KADpSDNEVpTDGEAKETK(A)
T-482 CKII 0.72 (K)KADSDNEVpTDGEAKETK(A)
(K)KADpSDNEVpTDGEAKETK(A)
S-502 PKA 0.69 (R)KPpSKAVKLDKEEVAAAETEAK(A)
  1. The analysis was performed with the software NetPhosK 1.0 [55]