Table 7 Strains and plasmids used in this work
From: Engineering of a microbial coculture of Escherichia coli strains for the biosynthesis of resveratrol
Strain | Characteristics | Reference |
|---|---|---|
W3110 | E. coli F−, λ−, INV (rrnD− rrnE)1 | ATCC 27325 |
W (pheA−)Rg | W3110 (pheA::Km) transformed with plasmid pJLBaroGfbr tktA and pTrcPALRg | [21] |
W-Ah | W3110 transformed with plasmid pTrc-Sc4CL-AhSTS | This work |
W-Vv | W3110 transformed with plasmid pTrc-Sc4CL-VvSTS | This work |
Plasmids | ||
pMK-RQ-Sc4CL | Sc4CL synthetized with optimal codon usage for E. coli | This work |
pMA-AhSTS | AhSTS synthetized with optimal codon usage for E. coli | This work |
pSB3K3-VvSTS | VvSTS synthetized with codon harmonization for E. coli | This work |
pTrc99A | Cloning vector carries bla and lacIq genes and trc promoter | [32] |
pJLBaroGfbrtktA | aroG fbr under control of the lacUV5 promoter; and tktA under its native promoter, carries lacIq and tet genes | [33] |
pTrcPALRg | PAL gene from R. glutinis gene cloned in pTrc99A | [21] |
pTrc-Sc4CL-AhSTS | 4CL gene from S. coelicolor A2 (Sc) and STS gene from A. hypogaea (Ah) cloned in pTrc99A | This work |
pTrc-Sc4CL-VvSTS | 4CL gene from S. coelicolor A2 (Sc) and STS gene from V. vinifera (Vv) cloned in pTrc99A | This work |