From: Engineering of a microbial coculture of Escherichia coli strains for the biosynthesis of resveratrol
Microorganism | Precursor | Time of culture/media | Genes introduced | Genes origin | Production (mg/L) | Reference |
---|---|---|---|---|---|---|
E. coli | p-coumaric acid | 24 h, 2 × YT | 4CL, STS | N. tabacum (4CL), V. vinifera (STS) | 16 | [29] |
E. coli | p-coumaric acid | 19 h, M9 + yeast extract + glycerol | 4CL, STS | A. thaliana (4CL), A. hypogaea (STS) | 104.5 | [8] |
E. coli | p-coumaric acid | 72 h, minimal MOPS medium + glucose | 4CL, STS | S. coelicolor (4CL), V. vinifera (STS) | 3.6 | [30] |
E. coli | p-coumaric acid | 60 h, M9 + yeast extract + glucose | 4CL, STS | Lithospermum erythrohizon (4CL), A. hypogaea (STS) | 171 | [31] |
E. coli | p-coumaric acid | 24 h, M9 + yeast extract + glycerol, cerulenin | 4CL, STS | A. thaliana (4CL), V. vinifera (STS) | 2340 | [13] |
E. coli | l-tyrosine, malonate | 48 h, minimal MOPS medium + glucose | TAL, 4CL, STS, MatB, MatC | R. glutinis (TAL), Petroselinum crispum (4CL), V. vinifera (STS), R. trifoli (matB, matC) | 35 | [9] |
E. coli | p-coumaric acid | 20 h, M9 + glycerol | 4CL, STS | S. coelicolor (4CL), V. vinifera (STS) | 78.1 | This work |
E. coli/E.coli pheA- (Coculture) | 20 h, M9 + glycerol + l-Phe | TAL, 4CL, STS | R. glutinis (TAL), S. coelicolor (4CL), V. vinifera (STS) | 22 | This work |