Table 3 Deracemization of 2-hydroxy acids with the mixtures of E. coli BL21(DE3)/pET28b-HADH and E. coli BL21(DE3)/pCDFDuet-KAR-GDH
Entry | Substrate | Reaction time (h) | Conversion (%) | ee of (R)-1 (%) |
|---|---|---|---|---|
1 | rac-1a | 4 | 94.9 | >99 |
2 | rac-1b | 6 | 94.5 | >99 |
3 | rac-1c | 6 | 93.2 | >99 |
4 | rac-1d | 4 | 95.1 | >99 |
5 | rac-1e | 4 | 96.4 | >99 |
6 | rac-1f | 4 | 97.3 | >99 |
7 | rac-1g | 4 | 97.6 | >99 |
8 | rac-1h | 2 | 95.5 | >99 |
9 | rac-1i | 4 | 96.8 | >99 |
10 | rac-1j | 2 | 94.5 | >99 |
11 | rac-1k | 4 | 96.3 | >99 |
12 | rac-1l | 2 | 96.4 | >99 |
13 | rac-1m | 4 | 94.8 | >99 |
14 | rac-1n | 6 | 35.5 | 81.9 |
15 | rac-1o | 6 | 34.6 | 71.8 |
16 | rac-1p | 6 | 59.5 | >99 |
17 | rac-1q | 6 | 42.7 | >99 |
18 | rac-1r | 6 | 76.4 | 63.3 |
19 | rac-1s | 6 | 58.3 | 60.1 |