Table 2 Reduction of keto acids to corresponding (R)-2-hydroxy acids by resting cells of E. coli BL21(DE3)/pCDFDuet-KAR-GDH
Entry | Substrate | Reaction time (h) | Conversion (%) | ee of (R)-1 (%) |
|---|---|---|---|---|
1 | 2a | 3.5 | 95.4 | >99 |
2 | 2b | 5 | 97.0 | >99 |
3 | 2c | 6 | 92.8 | >99 |
4 | 2d | 7 | 89.3 | >99 |
5 | 2e | 6 | 90.2 | >99 |
6 | 2f | 6 | 99.0 | >99 |
7 | 2g | 8 | 90.0 | >99 |
8 | 2h | 10 | 87.4 | >99 |
9 | 2i | 9 | 96.4 | >99 |
10 | 2j | 7 | 91.5 | >99 |
11 | 2k | 7 | 86.9 | >99 |
12 | 2l | 8 | 87.2 | >99 |
13 | 2m | 7 | 88.5 | >99 |
14 | 2n | 12 | 26.8 | >99 |
15 | 2o | 12 | 22.6 | >99 |
16 | 2p | 9 | 76.4 | >99 |
17 | 2q | 11 | 25.5 | >99 |
18 | 2r | 12 | 21.5 | >99 |
19 | 2s | 13 | 14.0 | >99 |