Fig. 2From: Enantioselective cascade biocatalysis for deracemization of 2-hydroxy acids using a three-enzyme systemTime course of deracemization of rac-1a with a single recombinant E. coli strain. The freshly prepared cells of E. coli BL21(DE3)/pET28b-HADH/pCDFDuet-KAR-GDH were resuspended in 10 mL phosphate buffer (100 mM, pH 7.5) to a cell concentration of 8 g DCW/LBack to article page