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Table 3 Strains, plasmids, and primers used in this study

From: Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica

Yeast strains

Genotype

Source

W29

Wild-type strain

[30]

JMY2033

W29 ura3::LEU2ex-zeta, leu2-270, xpr2-322

[39]

JMY2656

JMY2033 pPOX2-RedStar2

This study

JMY3742

JMY2033 pLIP2-RedStar2

This study

E. coli strain

Plasmid description

 

JME509

JMP114

[35]

JME803

JMP62

[40]

JME1394

JMP62-type LEU2ex-pTEF-RedStar2

[41]

JME1491

JMP62-type URA3ex-pTEF-RedStar2

This study

JME1500

JMP62-type URA3ex-pPOX2b-RedStar2

This study

JME1945

JMP62-type URA3ex-pLip2-RedStar2

This study

Primers

Sequence (5′–3′)

Restriction site

LAM51

ACTTTCGAAATGATAACGTTGGAGAAGAG

BstBI

LAM52

TCGGATCCTGAGGAGAGCTGGTACTTGG

BamHI

Lip2Qfo

CGAGGAACCCACTCTCTGG

 

Lip2Qrev

GCTCAATCACAGAGTCGAGC

 

RedStarQfo

CGAAGGTGAAGGTGAGGGTAG

 

RedStarQrev

CCACTTGAAACCTTCTGGGAAG

 

ACT-R

GGCCAGCCATATCGAGTCGCA

 

ACT-F

TCCAGGCCGTCCTCTCCC

 

POX2fo

GCCTGCATTCGACGACAGTTCTC

 

POX2rev

CGTCGGAAACAACCTCATTGAGGC

 

PGKfo

CAGGTGAGTACCCACGAAACGC

 

PGKrev

GCATCCCAATTTGCCTTAGCATTTG

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