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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica

Fig. 5

Glucose transport activity and intracellular lipid accumulation in individual cells in JMY3742 cultures grown in CMD, CMOA, and CMDOA. At the mid-log phase, cells were harvested, washed, and resuspended in a solution containing 2-NDBG (b) or Bodipy (c) to characterize glucose transport and fatty-acid accumulation, respectively. Levels of green fluorescence in 40,000 cells were analyzed after a 20-min incubation period using the FL1-A channel of a flow cytometer. Control strains without any straining are shown in a. Gates R1 to R4 are described in the “Methods” section. Media abbreviations are as in Fig. 1

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Microbial Cell Factories

ISSN: 1475-2859

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