Systems metabolic engineering of Corynebacterium glutamicum for the production of the carbon-5 platform chemicals 5-aminovalerate and glutarate

Table 3 Description of primers that were used in the present work for genome-based integration of tuf-promoter controlled davBA genes from P. putida in the bioD locus (PR_davBA_1–PR_davBA_10) and for deletion of the gabT gene (PR_gabT_1–PR_gabT_4) in C. glutamicum

No.	Sequence	AT [°C]
PR_davBA_1	CTGCGTTAATTAACAATTGGTAAGCAATGGCCTACAACCAGAC	59
PR_davBA_2	CATTCGCAGGGTAACGGCCAGGTTTATTTCCCTTTAACTGCAGC	57
PR_davBA_3	CAGTTAAAGGGAAATAAACCTGGCCGTTACCCTGCGAATG	62
PR_davBA_4	TGGCGGTTCTTCTTGTTCATTGTATGTCCTCCTGGACTTCGTGGTG	64
PR_davBA_5	GAAGTCCAGGAGGACATACAATGAACAAGAAGAACCGCCACCCCGC	68
PR_davBA_6	GCGCATTGTATGTCCTCCTGGACTTCTCAATCCGCCAGGGCGATCGG	66
PR_davBA_7	CGATCGCCCTGGCGGATTGAGAAGTCCAGGAGGACATACAATGCGCATCGCTCTGTACCAGG	64
PR_davBA_8	CGAAGGCACGGTGTTCACGATCAGCCTTTACGCAGGTGCAG	62
PR_davBA_9	TGCACCTGCGTAAAGGCTGATCGTGAACACCGTGCCTTCG	62
PR_davBA_10	AATCCCGGGTCTAGAGGATCACGCATGAGTGTGCTTGTGGAA	62
PR_gabT_1	CTGCGTTAATTAACAATTGGCGCTGGAGGTGATCGAGATAAATG	59
PR_gapT_2	CTGGGAAGGTCAAAGACACTGGTTCCTCCTGTGAGGTGAGATAC	59
PR_gabT_3	CTCACCTCACAGGAGGAACCAGTGTCTTTGACCTTCCCAG	56
PR_gabT_4	AATCCCGGGTCTAGAGGATCCAAAGACGGAGGCGATGATC	57

ISSN: 1475-2859