Table 2 Description of the C. glutamicum strains and plasmids used in the present work for the heterologous production of 5-aminovalerate and glutarate
Description | Reference | |
|---|---|---|
Strain | ||
C. glutamicum LYS-12 | Lysine-hyperproducing strain with 12 genome-based modifications | [34] |
C. glutamicum AVA-1 | LYS-12 + genome-based integration of the P. putida genes davB (PP_0383) and davA (PP_0382) encoding lysine monooxygenase and aminovaleramide amidase, into the bioD gene locus (NCgl2516), encoding dithiobiotin synthetase | This work |
C. glutamicum AVA-2 | AVA-1 + deletion of lysE gene (NCgl1214), encoding the lysine exporter | This work |
C. glutamicum AVA-3 | AVA-2 + deletion of putative gabT gene (NCgl0462), encoding 5-aminovalerate transaminase | This work |
Plasmids | ||
pTC | Expression vector for DNA-methyltransferase of C. glutamicum, ORI for E. coli and tetracycline resistance as selection marker. Used in E. coli NM522 to add the C. glutamicum-specific DNA-methylation pattern to the integrative transformation vector | [34] |
pClik int sacB | Integrative transformation vector for C. glutamicum with MCS, ORI for E. coli, and KanR and sacB as selection markers | [58] |
pClik int sacB tuf p -davBA | Integrative transformation vector for genome-based implementation of davBA genes from P. putida KT2440 into the bioD locus of C. glutamicum | This work |
pClik int sacB ∆lysE | Integrative transformation vector for deletion of the lysine exporter lysE | [12] |
pClik int sacB ∆gabT | Integrative transformation vector for deletion of the 5-aminovalerate transaminase gene gabT | This work |