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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Localization of a red fluorescence protein adsorbed on wild type and mutant spores of Bacillus subtilis

Fig. 6

Immuno-fluorescence microscopy of wild type and mutant spores adsorbed with mRFP. Immuno reactions were performed with anti-His primary antibody and fluorescent anti-mouse secondary antibody conjugated with FITC (“Methods” section). For each strain the same microscopy field was observed by phase contrast and fluorescence microscopy. Merge panels are reported. The exposure time is indicated as ms. The numbers in the panels are the percentage of spores found with a similar mRFP-localization pattern in three different microscopy fields. Scale bar 1 μm

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