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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Localization of a red fluorescence protein adsorbed on wild type and mutant spores of Bacillus subtilis

Fig. 2

(a) Dot blot of serial dilutions of unbound mRFP after adsorption reactions with wild type and cotH mutant spores. The amount of purified recombinant mRFP (ng) and the volume of supernatant loaded are indicated. Immuno reactions were performed with anti-His primary antibody conjugated with the horseradish peroxidase (“Methods” section). b Efficiency of adsorption (%) of wild type and mutant spores (black and white squares, respectively) with various amounts of mRFP. The graph is designed on the base of the averages from densitometric analysis of three independent dot blots, reported in Additional file 2: Table S1. Error bars show the standard errors of the mean from the three experiments (P = 0.0025)

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