Fig. 1From: Localization of a red fluorescence protein adsorbed on wild type and mutant spores of Bacillus subtilis Western (a) and dot (b) blot of proteins extracted from wild type and cotH mutant spores after mRFP-spore adsorption. Purified recombinant mRFP is used as marker. In panel b the amount of purified recombinant mRFP (ng) and the number of spores present in each dilution are indicated. Immuno reactions were performed with anti-His primary antibody conjugated with horseradish peroxidase ("Methods'' section)Back to article page