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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Lactococcus lactis carrying the pValac eukaryotic expression vector coding for IL-4 reduces chemically-induced intestinal inflammation by increasing the levels of IL-10-producing regulatory cells

Fig. 1

Schematic representation of the eukaryotic expression vector pValac::dts::IL-4 and analysis of its functionality. a Schematic representation of pValac::dts::IL-4 showing the DTS (yellow), pCMV promoter, IL-4 ORF (blue), E. coli repC and L. lactis repA origins of replication, and the ORF that confers resistance to the antibiotic chloramphenicol (CmR). b Photograph of (I) non-transfected CHO cells and cells transfected with (II) pValac::dts and (III) pValac::dts::IL-4 marked with DAPI (Life Technologies; Carlsbad, CA/USA), rat anti-mouse IL-4 (Santa Cruz Biotechnology, Inc.; Dallas, TX/USA), and rabbit anti-rat IgG conjugated with Alexa Fluor 488 (Life Technologies; Carlsbad, CA/USA) antibodies showing the cell nucleus in blue and the murine IL-4 in green. c Flow cytometry acquired events showing (I) non-transfected CHO cells and cells transfected with (II) pValac::dts and (III) pValac::dts::IL-4 marked with APC rat anti-mouse IL-4 antibody (BD; San Jose, CA/USA). The positive events lie within the rectangles, together with their percentages. DTS DNA nuclear targeting sequence; pCMV cytomegalovirus promoter; IL-4 interleukin 4; ORF open reading frame; CHO chinese hamster ovary cells

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