Fig. 5
Growth and lipid accumulation by Y. lipolytica strains in optimized conditions. JMY2900 (control), JMY3675 (SUC+), JMY1631 (DGA−), JMY3400 (DGA2 × 1), JMY3357 (DGA2 × 2), and JMY3850 (DGA2 × 4) were cultured in YNB0.85Nc1.5G30.6 medium at a C/N ratio of 35. Growth was monitored in a 48-well flowerplate (filling volume: 800 µL, temperature: 28 °C, agitation: 1200 rpm) by measuring scattered light (ex: 620 nm/em: -, Gain: 10), and total lipids were monitored using Bodipy and the GFP optical filter (ex: 486 nm/em: 508 nm; Gain = 80). a was plotted by transforming units of scattered light into CDW (X in g L−1; line) and X in Ln(X). b The lipid yield was evaluated using Bodipy fluorescence and biomass (CDW). c Total lipid content and d maximal specific lipid production rate of the yeast strains