Fig. 2
Growth of Y. lipolytica using different carbon sources in a rich medium. Y. lipolytica JMY3675 was cultured in rich medium that was supplemented with either glucose (D; Fig. 2a), sucrose (S; Fig. 2b), or glycerol (G; Fig. 2c). The growth of this strain was monitored in a 48-well flowerplate (filling volume: 800 µL, temperature: 28 °C, agitation: 1200 rpm) by measuring scattered light (ex: 620 nm/em: -, Gain: 10). In each medium, the sugars and glycerol were mixed with peptones and yeast extract to reach a C/N ratio of 15, 25, 35, or 45, as indicated in the legends. The figure was plotted by transforming units of scattered light into CDW (X in g L−1; line). d The specific growth rate of the strain was determined for each carbon source and initial carbon concentration. e Intracellular lipid yield and f total lipids at 110 h of culture were evaluated by measuring Bodipy fluorescence