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Fig. 1 | Microbial Cell Factories

Fig. 1

From: High-throughput fermentation screening for the yeast Yarrowia lipolytica with real-time monitoring of biomass and lipid production

Fig. 1

Evaluation of Y. lipolytica biomass monitoring with scattered light and lipid accumulation with fluorescent dyes. a The limits of biomass monitoring using scattered light were evaluated by comparing scattered light measurements with those of CDW or OD600nm. b The ability of two probes (Nile red and Bodipy) to measure total lipid production was evaluated using 1 µg/mL of both probes in a defined medium that contained 0.85 g L−1 of YNB, 1.5 g L−1 of NH4Cl, and 30.6 g L−1 of glycerol. c In each well, 1 µg/mL of the intracellular lipophilic probe Bodipy (ex: 486 nm/em: 508 nm) was added and the limit of intracellular lipid monitoring with fluorescence detection was evaluated. Scattered light (ex: 620 nm/em: -, Gain: 10), Bodipy fluorescence (ex: 488 nm/em: 520 nm, Gain: 80), and Nile red fluorescence (ex: 589 nm/em: 671 nm, Gain: 80) were measured using a 48-well flowerplate with 800 µL filling volume at 28 °C and 1200 rpm (shaking diameter: 3 mm)

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