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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Highly active promoters and native secretion signals for protein production during extremely low growth rates in Aspergillus niger

Fig. 5

thp mRNA analysis throughout a time-course of perfusion cultivation. a Northern blot analysis of mRNA extracted from strains MA237 (Panafp::thp) and MA238 (PhfbD::thp) during a time-course of perfusion fermentation are shown, with probes for THP mRNA (upper panel) and actin mRNA (middle panel). These data demonstrate thp mRNA is expressed in this system using both anafp and hfbD promoters. The lower panel demonstrates representative decrease of 18S and 28S rRNA on Northern blot membranes throughout the experiment time course, which we hypothesize is due to decrease in cellular metabolism. b Quantification of mRNA abundance from Northern blot results of thp (a) in strain MA237 (Panafp::thp) and strain MA238 (PhfbD::thp). These data suggest highest mRNA expression after 4 days of perfusion cultivation in isolate MA237, and approximately constitutive expression between days 4 until day 12 in isolate MA238

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Microbial Cell Factories

ISSN: 1475-2859

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