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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Highly active promoters and native secretion signals for protein production during extremely low growth rates in Aspergillus niger

Fig. 2

Biomass accumulation and specific growth rate. Biomass and specific growth rates (µ) are reported after a switch from batch to perfusion cultivation (day 0). Biomass values are reported as unconnected points, whereas growth rates are reported with connecting lines. The biomass concentration increased during the biomass retention from ~2.1 gdry weight/kg up to ~12 gdry weight/kg in 12 days. The strain FW23.7 (Panafp::afp) has the double amount of glucose available (batch: 0.8 % glucose, perfusion: 1.6 % glucose), resulting in an approximately ~6 h earlier switch to perfusion cultivation, and a final biomass after 12 days of ~20 gdry weight/kg. The right diagram axis shows strain specific growth rate, which decreased within 2 days of changing cultivation conditions and reached values close to zero. For each strain one single bioreactor run was performed. N402 data taken from [9]

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