Table 3 Primers used in this study
Primer name | Primer sequence | Notes |
|---|---|---|
AA_B1_BstB1_BFP_fw | AAGTTCGAAACGATGTCTGAATTGATTAAAGAGAATATG | |
AA_B3_EcoR1_BFP_rev | AGGGAATTCTCATTAGTTCAATTTGTGTCCTAACTTA | |
AB_A8_AOX1end_Rv | CGTTTCGAATAATTAGTTGTTTTTTGATCTTCT | |
AB_A9_pARS1_GOIend_Fw | GAATTCCAACCTGCGATTGATCT | |
qPCR-PpACT1-Fw (ACT1, Set 1) | CCATCCATTGTGCACCTCAAG | [35] |
qPCR-PpACT1-Rv (ACT1, Set 1) | CGTCTAGAAGCTGAACGACAAG | [35] |
qPCR-PpACT1_2_Fw (ACT1, Set 2) | CGAATCTGGACCATCCATTGTG | |
qPCR-PpACT1_2_Rv (ACT1, Set 2) | ACGACAAGTAGACACCACCAATC | |
qPCR_pEM7_1_Fv (Zeo, Set 1) | ACGACAAGGTGAGGAACTAAACC | |
qPCR_ZEO_1_Rv (Zeo, Set 1) | AAGTCGTCCTCCACGAAGTC | |
qPCR_pEM7_2_Fv (Zeo, Set 2) | CGACAAGGTGAGGAACTAAACC | |
qPCR_ZEO_2_Rv (Zeo, Set 2) | GAAGTCGTCCTCCACGAAGTC | |
qPCR_BFP_1_Fw (BFP, Set 1) | AGACGGTGGAGTTTTGACTGC | |
qPCR_BFP_1_Rv (BFP, Set 1) | AATGTCTCAGTGAATGCCTCCC | |
qPCR_BFP_2_Fw (BFP, Set 2) | ACGGTGGAGTTTTGACTGCTAC | |
qPCR_BFP_2_Rv (BFP, Set 2) | TGAATGCCTCCCATCCCAATG |