|
Fragments
|
Molar ratio
|
Overlapping ends (nts)
|
Fluorescent coloniesa
|
Coefficient of variation
|
Fluorescent colonies (%)b
|
|---|
|
1 + 2 + 3
|
1:1:1
|
50
|
47
|
2.13
|
65.3
|
|
1 + 2 + 3
|
1:1:1
|
20
|
42.5
|
3.53
|
59
|
|
1 + 2 + 3
|
1:1:3
|
50
|
62.5
|
4
|
86.8
|
|
1 + 2 + 3
|
1:1:3
|
20
|
63
|
4.76
|
87.5
|
|
1 + 2 + 3
|
1:1:6
|
50
|
65.5
|
2.29
|
91
|
|
1 + 2 + 3
|
1:1:6
|
20
|
65
|
3.07
|
90.3
|
-
aAverage of colony numbers obtained from 3 independent transformations
-
bClones are defined as positive if their normalized fluorescent resulted >80 % of the difference between positive and negative controls
