Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

Table 2 Summary of results for in vivo recombination (3-fragments assembly)

Fragments	Molar ratio	Overlapping ends (nts)	Fluorescent colonies^a	Coefficient of variation	Fluorescent colonies (%)^b
1 + 2 + 3	1:1:1	50	47	2.13	65.3
1 + 2 + 3	1:1:1	20	42.5	3.53	59
1 + 2 + 3	1:1:3	50	62.5	4	86.8
1 + 2 + 3	1:1:3	20	63	4.76	87.5
1 + 2 + 3	1:1:6	50	65.5	2.29	91
1 + 2 + 3	1:1:6	20	65	3.07	90.3

^aAverage of colony numbers obtained from 3 independent transformations
^bClones are defined as positive if their normalized fluorescent resulted >80 % of the difference between positive and negative controls

ISSN: 1475-2859