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Table 2 Summary of results for in vivo recombination (3-fragments assembly)

From: Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

Fragments Molar ratio Overlapping ends (nts) Fluorescent coloniesa Coefficient of variation Fluorescent colonies (%)b
1 + 2 + 3 1:1:1 50 47 2.13 65.3
1 + 2 + 3 1:1:1 20 42.5 3.53 59
1 + 2 + 3 1:1:3 50 62.5 4 86.8
1 + 2 + 3 1:1:3 20 63 4.76 87.5
1 + 2 + 3 1:1:6 50 65.5 2.29 91
1 + 2 + 3 1:1:6 20 65 3.07 90.3
  1. aAverage of colony numbers obtained from 3 independent transformations
  2. bClones are defined as positive if their normalized fluorescent resulted >80 % of the difference between positive and negative controls