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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

Fig. 6

Fragment ratio determination for integrative, episomal and in vivo recombined plasmids (three fragments assembly). Cluster plot for Zeo/BFP fragment ratio for 4 independent clones carrying integrative (red dot), episomal (orange diamond) or three-fragments in vivo recombined (1:1:1 lime square; 1:1:3 green triangle—3 independent clones; 1:1:6 blue inverted triangle). All distributions were evaluated using one-factor ANOVA analysis (α 0.05); the average distribution between sample 1:1:1 and 1:1:6 (bracketed and indicated by a star) resulted statistically significant by ANOVA test

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