Fig. 6From: Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applicationsFragment ratio determination for integrative, episomal and in vivo recombined plasmids (three fragments assembly). Cluster plot for Zeo/BFP fragment ratio for 4 independent clones carrying integrative (red dot), episomal (orange diamond) or three-fragments in vivo recombined (1:1:1 lime square; 1:1:3 green triangle—3 independent clones; 1:1:6 blue inverted triangle). All distributions were evaluated using one-factor ANOVA analysis (α 0.05); the average distribution between sample 1:1:1 and 1:1:6 (bracketed and indicated by a star) resulted statistically significant by ANOVA testBack to article page