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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

Fig. 4

Proof-of-principle of in vivo recombination (two-fragment assembly). a Schematic diagram of fragments used for testing in vivo recombination using pARS-BFP as template. b Frequencies of transformation obtained using overlaps of 100, 50, or 20 nucleotides between fragment 1 and fragments 2, 3, or 4. c BFP fluorescence signal obtained from 8 independent colonies randomly selected from the different pools (ns non-significant, according to Sidak’s multiple comparison test on one-way ANOVA, α 0.05)

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Microbial Cell Factories

ISSN: 1475-2859

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