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Table 2 Kinetic parameters for oxidation of (R)-PED to 2-HAP by RCR and reduction of 2-HAP to (S)-PED by SCR in recombinant C. parapsilosis/pCP-rcr and wild-type C. parapsilosis

From: In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of racemic mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011

Strains

Oxidation of (R)-PED to 2-HAP by RCR

Reduction of 2-HAP to (S)-PED by SCR

Relativea k cat /K m

K m (mM)

k cat (S−1)

k cat /K m (S−1 mM−1)

K m (mM)

k cat (S−1)

k cat /K m (S−1 mM−1)

C. parasilosis

6.53 ± 0.20

1.88 ± 0.14

0.29

4.36 ± 0.13

2.58 ± 0.05

0.59

2.03

C. parapsilosis/pCP-rcr

6.31 ± 0.09

3.19 ± 0.22

0.51

4.39 ± 0.10

2.53 ± 0.02

0.58

1.14

  1. All reactions involved in the calculation of kinetics constants were assayed with 100 mM acetate buffer (pH 6.5) at 35 °C. All experiments were repeated three to five times
  2. aRelative k cat /K m between oxidation of (R)-PED to 2-HAP by RCR and reduction of 2-HAP to (S)-PED by SCR
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Microbial Cell Factories

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