In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of racemic mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011

Table 1 The specific activities of RCR and SCR in the cell-free extracts of recombinant C. parapsilosis/pCP-rcr and wild-type C. parapsilosis

Cell-free extracts	RCR^a		SCR	Biotransformation
Cell-free extracts	Oxidative activities (U/mg) (R)-PED→2-HAP	Reductive activities (U/mg) 2-HAP→(R)-PED	Reductive activities (U/mg) 2-HAP→(S)-PED	Optical purity (%)	Yield (%)	Duration (h)
C. parapsilosis	0.04 ± 0.00	0.16 ± 0.08	0.39 ± 0.03	92.5 ± 1.7	32.8 ± 0.8	48
C. parapsilosis/pCP-rcr	0.15 ± 0.01	0.74 ± 0.07	0.40 ± 0.04	95.6 ± 2.1	43.5 ± 1.0	30

^aThe reductive activities of RCR catalyzing (R)-PED to 2-HAP were determined using NADH as cofactor. The oxidative activities of RCR catalyzing 2-HAP to (R)-PED were determined using NAD⁺ as cofactor

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