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Fig. 3 | Microbial Cell Factories

Fig. 3

From: In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of racemic mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011

Fig. 3

a SDS-PAGE analysis of RCR expression at different time points in recombinant C. parapsilosis/pCP-RCR. The cell-free extracts of C. parapsilosis/pCP-RCR collected at different time points were separated on 15 % polyacrylamide gels. Lane M, protein molecular standards. b SDS-PAGE analysis of purified RCR. ReQ the fraction purified by Resource Q chromatography; S200 the fraction purified by Superdex-200 chromatography; SCR purified SCR from C. parapsilosis/pCP-RCR; Lane M, protein molecular standards

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