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Fig. 2 | Microbial Cell Factories

Fig. 2

From: In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of racemic mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011

Fig. 2

Construction of an expression cassette for RCR in C. parapsilosis. ura3p (upstream sequence of ura3 gene) and ura3t (downstream sequence of ura3 gene) fragments were amplified using primers URA3p_1/URA3p_2 and URA3t_1/URA3t_2, and used as homologous regions for insertion into the genome. mal2p (upstream sequence of mal2 gene) and act1p (upstream sequence of act1 gene) fragments were amplified using primers MAL2p_1/MAL2p_2 and ACT1p_1/ACT1p_2, and used as the promoters for expression of the rcr and sat1 genes, respectively. act1t (downstream sequence of act1 gene) and ura3t (downstream sequence of ura3 gene) fragments were amplified using primers ACT1t_1/ACT1t_2 and URA3t_1/URA3t_2, and used as the terminators for the rcr and sat1 genes, respectively. Restriction sites are unique. E EcoRI, B BglII, S SacI, K KpnI, P PstI, N NotI, BH BamHI, H Hind III

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Microbial Cell Factories

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