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Fig. 1 | Microbial Cell Factories

Fig. 1

From: In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of racemic mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011

Fig. 1

The stereoinversion of, (R,S)-PED to its (S)-isomer by RCR and SCR from C. parapsilosis. An NAD+-dependent RCR catalyzes the reversible reaction between (R)-PED and 2-HAP. An NADPH-linked SCR transforms the intermediate 2-HAP to (S)-PED. The oxidation of (R)-PED to 2-HAP is rate-limiting step during the stereoinversion of racemic (R,S)-PED to its (S)-isomer. The desired product (S)-PED is underlined. PED, 1-phenyl-1,2-ethanediol; RCR, (R)-carbonyl reductase; SCR, (S)-carbonyl reductase

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