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Table 1 Km and Vmax values of rGAPDH purified from different host strains

From: Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

Host

Km (G3P) (µM)

Vmax (G3P) (min−1)

Km (NAD+) (µM)

E. coli BL21DE3 13

280 ± 30

1670 ± 90

 
 

40 ± 4

  

M. smegmatis

223.8 ± 4.66

0.128 ± 0.0023

109.4 ± 3.4

M.tb H37Ra

169.6 ± 10.53

0.199 ± 0.012

101.2 ± 8.9

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Microbial Cell Factories

ISSN: 1475-2859

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