Fig. 6
Localization, purification and activity recombinant M.tb Enolase in different host strains. a–c Localization of rH-Enolase in cellular fractions of E. coli BL21DE3 GroEL/ES, M. smegmatis and M.tb H37Ra strains respectively, detection was done using α-His antibodies. d Affinity purification of EC-H-Eno, analysis by 10 % SDS-PAGE e Western blotting with α-His antibody. f Affinity purification of MS-H-Eno), analysis by 10 % SDS-PAGE. g Western blotting with α-His antibodies. h Affinity purification of MT-H-Eno, analysis by 10 % SDS-PAGE. i Western blotting with α-His antibody. Residual enzyme activity (in %) of EC-H-Eno, MS-H-Eno and MT-H-Eno j in the absence of Mg2+, k in the presence of Mg2+. Significance was determined by student’s unpaired t test where *p < 0.05; **p < 0.01 and ***p < 0.001