Fig. 2

Localization and purification of recombinant N-terminal His tagged M.tb GAPDH from M. smegmatis and M.tb H37Ra strains: Localization of rH-GAPDH in cellular fractions of M. smegmatis detection was done using a α-GAPDH and b α-His antibodies. Localization of rGAPDH in cellular fractions of M.tb H37Ra detection was done using c α-GAPDH and d α-His antibodies. e Affinity purification of MS-H-GAPDH, analysis by 10 % SDS-PAGE. f Western blot with α-GAPDH and α-His to confirm purification from M. smegmatis. g Affinity purification of MT-H-GAPDH. h Western blot with α-GAPDH and α-His to confirm purification from M. tb H37Ra. i Comparative enzyme activity of N- (MT-H-GAPDH) and C-terminal (MT-GAPDH-H) His tagged recombinant GAPDH purified from M.tb H37Ra. Inset: Comparison of end point values, experiment was repeated three times (n = 3), data is represented as absorbance at 340 nm ±SD