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Table 1 Strains and plasmids used in this study

From: Engineering Saccharomyces cerevisiae with the deletion of endogenous glucosidases for the production of flavonoid glucosides

Strains or plasmids

Relevant properties

Source or reference

Strains

 E. coli trans1-T1

F− φ80(lacZ)∆M15 ∆lacX74 hsdR(rK− mK+)∆recA1398 endA1 tonA

Our lab

 E. coli BL21 (DE3)

F− ompT hsdS (rBB-mB −) gal dcm

Our lab

 S. cerevisiae W303-1b

MATα ade2-1 leu2-3, 112 his3-11, 15 ura3-1 trp1-1

Our lab

 W303-1b/EXG1∆

W303-1b derivative with pEZ-EXG36-Trp1, EXG1∆::Trp1

This study

 W303-1b/SPR1∆

W303-1b derivative with pEZ-SPR36-ADE2, SPR1∆::Ade2

This study

 W303-1b/YIR007W∆

W303-1b derivative with pEZ-YIR36-Ura3, YIR007W∆::Ura3

This study

 W303-1b/ES∆

W303-1b derivative with pEZ-EXG36-Trp1 and pEZ-SPR36-ADE2, EXG1∆::Trp1, SPR1∆::Ade2

This study

 W303-1b/SbGT34

W303-1b derivative with pδGAPg-SbGT34

This study

 W303-1b/ES∆/SbGT34

W303-1b/ES∆ derivative with pδGAPg-SbGT34

This study

 W303-1b/ES∆/PU/SbGT34

W303-1b/ES∆ derivative with pδGAPg-PGM2, pδGAPh-UGP1, and pδGAPg-SbGT34

This study

Plasmids

 pTWIN1B

A derivative of pTWIN1, an inducible expression vector with ampicillin resistant.

Our lab

 pTWIN1B-SbGT30

pTWIN1B derivative with the SbGT30 gene DNA

This study

 pTWIN1B-SbGT34

pTWIN1B derivative with the SbGT34 gene DNA

This study

 pTWIN1B-SbGT56

pTWIN1B derivative with the SbGT56 gene DNA

This study

 pEZ-EXG36-TRP1

Integration vector with Homologous fragment of EXG1 and selective marker TRP1

This study

 pEZ-SPR36-ADE2

Integration vector with Homologous fragment of SPR1 and selective marker ADE2

This study

 pEZ-YIR36-URA3

Integration vector with Homologous fragment of YIR007W and selective marker URA3

This study

 pδGAPg

pBluescript II KS(+) derivative with homologous δ region, P gap , T pgk1 , G418r

[34]

 pδGAPh

pBluescript II KS(+) derivative with homologous δ region, P gap , T pgk1 , HygBr

[34]

 pδGAPg-PGM

pδGAPg derivative with PGM2 from S. cerevisiae, G418r

This study

 pδGAPh-UGP1

pδGAPh derivative with UGP1 from S. cerevisiae, HygBr

This study

 pδGAPg-SbGT34

pδGAPg derivative with SbGT34 from S. baicalensis, G418r

This study