Strains or plasmids | Relevant properties | Source or reference |
---|---|---|
Strains | ||
 E. coli trans1-T1 | F− φ80(lacZ)∆M15 ∆lacX74 hsdR(rK− mK+)∆recA1398 endA1 tonA | Our lab |
 E. coli BL21 (DE3) | F− ompT hsdS (rBB-mB −) gal dcm | Our lab |
 S. cerevisiae W303-1b | MATα ade2-1 leu2-3, 112 his3-11, 15 ura3-1 trp1-1 | Our lab |
 W303-1b/EXG1∆ | W303-1b derivative with pEZ-EXG36-Trp1, EXG1∆::Trp1 | This study |
 W303-1b/SPR1∆ | W303-1b derivative with pEZ-SPR36-ADE2, SPR1∆::Ade2 | This study |
 W303-1b/YIR007W∆ | W303-1b derivative with pEZ-YIR36-Ura3, YIR007W∆::Ura3 | This study |
 W303-1b/ES∆ | W303-1b derivative with pEZ-EXG36-Trp1 and pEZ-SPR36-ADE2, EXG1∆::Trp1, SPR1∆::Ade2 | This study |
 W303-1b/SbGT34 | W303-1b derivative with pδGAPg-SbGT34 | This study |
 W303-1b/ES∆/SbGT34 | W303-1b/ES∆ derivative with pδGAPg-SbGT34 | This study |
 W303-1b/ES∆/PU/SbGT34 | W303-1b/ES∆ derivative with pδGAPg-PGM2, pδGAPh-UGP1, and pδGAPg-SbGT34 | This study |
Plasmids | ||
 pTWIN1B | A derivative of pTWIN1, an inducible expression vector with ampicillin resistant. | Our lab |
 pTWIN1B-SbGT30 | pTWIN1B derivative with the SbGT30 gene DNA | This study |
 pTWIN1B-SbGT34 | pTWIN1B derivative with the SbGT34 gene DNA | This study |
 pTWIN1B-SbGT56 | pTWIN1B derivative with the SbGT56 gene DNA | This study |
 pEZ-EXG36-TRP1 | Integration vector with Homologous fragment of EXG1 and selective marker TRP1 | This study |
 pEZ-SPR36-ADE2 | Integration vector with Homologous fragment of SPR1 and selective marker ADE2 | This study |
 pEZ-YIR36-URA3 | Integration vector with Homologous fragment of YIR007W and selective marker URA3 | This study |
 pδGAPg | pBluescript II KS(+) derivative with homologous δ region, P gap , T pgk1 , G418r | [34] |
 pδGAPh | pBluescript II KS(+) derivative with homologous δ region, P gap , T pgk1 , HygBr | [34] |
 pδGAPg-PGM | pδGAPg derivative with PGM2 from S. cerevisiae, G418r | This study |
 pδGAPh-UGP1 | pδGAPh derivative with UGP1 from S. cerevisiae, HygBr | This study |
 pδGAPg-SbGT34 | pδGAPg derivative with SbGT34 from S. baicalensis, G418r | This study |