Enhanced production of polyhydroxybutyrate by multiple dividing E. coli

Table 1 Cell growth and PHA accumulation by recombinant E. coli grown under different culture conditions

Culture medium	Strain	CDW (g/l)^a	PHA (wt%)^b
MM + 0.5 g/L yeast extract	JM109ΔminCD	4.96 ± 0.29	39.71 ± 1.72
MM + 0.5 g/L yeast extract	JM109	4.41 ± 0.16	36.53 ± 0.24
MM + 1 g/L yeast extract	JM109ΔminCD	5.12 ± 0.22	43.08 ± 0.37
MM + 1 g/L yeast extract	JM109	5.09 ± 0.28	40.81 ± 0.55
MM + 1.5 g/L yeast extract	JM109ΔminCD	5.09 ± 0.35	41.26 ± 1.15
MM + 1.5 g/L yeast extract	JM109	4.53 ± 0.24	38.19 ± 0.66

^aCells were cultivated in mineral medium (MM) added with various amounts of yeast extract containing 20 g/L glucose at 37 °C for 48 h
^bData are expressed as the M ± SD, M refers to a mean value and SD standard deviations (n = 3)

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