Fig. 5

Effect of ftsQLWN over-expression on cell morphology and PHA accumulation in E. coli JM109 ∆minCD. SEM morphology studies on: a E. coli JM109 (p15a-blank). b E. coli JM109 ∆minCD (p15a-blank). c E. coli JM109 ∆minCD (p15a-Pbad-ftsQLWN). Scale bars, 10 μm. d The cell growth rate of E. coli JM109 ∆minCD harboring plasmid p15a-Pbad-ftsQLWN.Strains were grown in the LB medium, and 0.2 % arabinose was added into the medium after 2 h of inoculation. Error bars are s.d. (n = 3). e The cell dry weight and PHA accumulation by E. coli JM109 ∆minCD (p15a-Pglta-ftsQLWN) with pBHR68, Cells were cultivated in LB mediums referred to containing 20 g/L glucose at 37 °C for 48 h. Error bars are s.d. (n = 3). f The cell growth and PHA production by E. coli JM109 ∆minCD (p15a-Pbad-ftsQLWN) with pBHR68. The strains were grown in the LB medium at 37 °C to an OD600 = 0.4–0.6 (2 h after injection), followed by induction with 0.2 % arabinose for 6 h, then addition of 20 g/l glucose for another 40 h cultivation. Error bars are s.d. (n = 3)