Table 1 Strains and plasmids used in this study
Strains and plasmids | Relevant gene type | Reference/source |
|---|---|---|
Strains | ||
DH5α | E. coli F−Ф80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (r −k m −k ) phoA supE44 thi −1 gyrA96 relA1 | Lab stock |
E.coli S17-1 (λpir) | res− pro mod+ integrated copy of RP4, mob+, used for incorporating constructs into P. chlororaphis | Hoffmann et al. [38] |
GP72 | P. chlororaphis GP72 wild-type strain | Lab stock |
GP72AN | rpeA insertionally inactivation mutat of GP72 | Lab stock |
GP72Δpyk | pykF in-frame deletion mutant of GP72 | This study |
GP72ΔrsmE | rsmE in-frame deletion mutant of GP72 | This study |
GP72Δlon | lon in-frame deletion mutant of GP72 | This study |
GP72ND-1 | rpeA in-frame deletion mutant of GP72Δpyk | This study |
GP72ND-2 | rsmE in-frame deletion mutant of GP72ND-1 | This study |
GP72ND-3 | lon in-frame deletion mutant of GP72ND-2 | This study |
GP72ND-3-pBbB5K-aroB | aroB overexpression in GP72ND-3 | This study |
GP72ND-3-pBbB5K-aroD | aroD overexpression in GP72ND-3 | This study |
GP72ND-3-pBbB5K-aroE | aroE overexpression in GP72ND-3 | This study |
GP72ND-3-pBbB5K-phzC | phzC overexpression in GP72ND-3 | This study |
GP72ND-3- pBbB5K-tktA | tktA overexpression in GP72ND-3 | This study |
GP72 ND-3- pBbB5K-ppsA | ppsA overexpression in GP72ND-3 | This study |
GP72 ND-3- pBbB5K-aroE-aroD | aroE and aroD overexpression in GP72ND-3 | This study |
GP72 ND-3-pBbB5K-aroE-aroD-aroB | aroE, aroD and aroB overexpression in GP72ND-3 | This study |
GP72 ND-3- pBbB5K-tktA-ppsA | tktA and ppsA overexpression in GP72ND-3 | This study |
GP72 ND-3- pBbB5K-phzC-tktA-ppsA | phzC, tktA and ppsA overexpression in GP72ND-3 | This study |
GP72 ND-3- pBbB5K-aroE-aroD-aroB-phzC-tktA-ppsA | aroE, aroD, aroB, phzC, tktA and ppsA overexpression in GP72ND-3 | This study |
Plasmids | ||
pMD19-T simple | T-Vector for gene coloning, Apr | Lab stock |
pEASY-Blunt | Blunt vector for gene coloning, Apr, Kanr | Lab stock |
pMD19-T-aroD | Site-specific mutant vector for gene aroD | This study |
pEASY-Blunt-tktA | Site-specific mutant vector for gene tktA | This study |
pEASY-Blunt-ppsA | Site-specific mutant vector for gene ppsA | This study |
pK18mobsacB | Broad-host-range gene replacement vector, sacB, Kanr | Schafer et al. [39] |
pK18-pyk | pK18mobsacB containing pykF flanking region | This study |
pK18-rsmE | pK18mobsacB containing rsmE flanking region | This study |
pK18-lon | pK18mobsacB containing lon flanking region | This study |
pBbB5K-GFP | pBBR1; Knr lacI Plac-UV5 | Lee et al. [40] |
pBbB5K-aroB | Plasmid for aroB overexpression | This study |
pBbB5K-aroD | Plasmid for aroD overexpression | This study |
pBbB5K-aroE | Plasmid for aroE overexpression | This study |
pBbB5K-phzC | Plasmid for phzC overexpression | This study |
pBbB5K-tktA | Plasmid for tktA overexpression | This study |
pBbB5K-ppsA | Plasmid for ppsA overexpression | This study |
pBbB5K-aroE-aroD | Plasmid for aroE, aroD overexpression | This study |
pBbB5K-aroE-aroD-aroB | Plasmid for aroE, aroD and aroB overexpression | This study |
pBbB5K-tktA-ppsA | Plasmid for tktA and ppsA overexpression | This study |
pBbB5K-phzC-tktA-ppsA | Plasmid for phzC, tktA and ppsA overexpression | This study |
pBbB5K-aroE-aroD-aroB-phzC-tktA-ppsA | Plasmid for aroE, aroD, aroB,phzC, tktA and ppsA, overexpression | This study |