Fig. 9
From: Rewiring a secondary metabolite pathway towards itaconic acid production in Aspergillus niger
Hypothetical pathway of itaconic acid production in citB overexpressing A. niger mutants. Hypothetical pathway in citB overexpressing IA producing strains. CitB has no known mitochondrial targeting sequence and is thought to be located in the cytosol where it converts acetyl-CoA and oxaloacetate into citrate. Citrate continues to be further processed by aconitase into cis-aconitate which eventually is decarboxylated by CadA into IA. Theoretically, cis-aconitate shuttling from mitochondria to cytoplasm by MttA is redundant in this novel pathway. Note that only direct pathway steps towards IA production are shown