Fig. 2From: Rewiring a secondary metabolite pathway towards itaconic acid production in Aspergillus niger Shake flask cultivation of itaconic acid producing A. niger transformants. HPLC analysis of shake flask experiment with AB1.13 itaconic acid producing transformants. Shake flasks were cultivated for 5 days prior to analysis. All strains were analyzed in triplicate. a Production of itaconic acid is compared amongst strains carrying CAD, CAD + MFS, CAD + MTT and CAD + MFS + MTT. Strains AB1.13 #49 and #63 have an fivefold improvement in IA production compared with the parental strain AB1.13 MFS 3.9. b Production of citric acid is compared amongst strains carrying CAD, CAD + MFS, CAD + MTT and CAD + MFS + MTT. The highest IA producers AB1.13 #49 and #63 produce the lowest amount of CA among all strains carrying the full IA biosynthesis clusterBack to article page