Fig. 2
CGQ growth curves in the context of typical offline data for CEN.PK2-1C grown on glucose as carbon source. Comparison of CGQ growth measurements and offline OD600, HPLC data of glucose and ethanol as well as microscopic images for S. cerevisiae CEN.PK2-1C on SC media with 1.5 % glucose. CGQ-backscatter readings were converted into OD600 using calibration files either for stationary cells or for exponentially growing cells. Yellow squares with purple edges indicate offline OD600 values being taken by the CGQuant software for correlation data refinement. Microscopic images depict representative snapshots of the current cell morphologies and sizes at the respective sampling time (a and b, as marked in the chart) and were all collected under the same magnification. The inset in microscopic image a depicts additionally 1.5-fold magnified cells from the same droplet during excessive budding