Table 1 Plasmids and strains used in this study
From: High production of fatty alcohols in Escherichia coli with fatty acid starvation
Plasmids/strains | Relevant characteristic(s) | Reference/source |
|---|---|---|
Plasmids | ||
pMD™ 18-T Vector | T-easy vector | Takara |
pTrcHisA | Expression vector | Invitrogen |
pKD46 | Bla γβexo temperature conditional pSC101 replicon | [8] |
pEASY-cat-sacB | T-easy vector with cat-sacB cassette | Lab collection |
pL1 | pTrcHisA containing the M. aquaeolei FAR gene | This study |
Strains | ||
W | E. coli K-12 MG1655 | Lab collection |
MGKC | E. coli K-12 MG1655 ΔtesC | This study |
MGKB | E. coli K-12 MG1655 ΔtesB | This study |
MGKA | E. coli K-12 MG1655 ΔtesA | This study |
MGKCB | E. coli K-12 MG1655 ΔtesC ΔtesB | This study |
MGKCBA | E. coli K-12 MG1655 ΔtesC ΔtesB ΔtesA | This study |
MGL1 | E. coli K-12 MG1655 ΔtesC ΔtesB ΔldhA::kan Δpta ΔackA | This study |
W/pL1 | W bearing pL1 | This study |
MGKC/pL1 | MGKC bearing pL1 | This study |
MGKB/pL1 | MGKB bearing pL1 | This study |
MGKA/pL1 | MGKA bearing pL1 | This study |
MGKCB/pL1 | MGKCB bearing pL1 | This study |
MGKCBA/pL1 | MGKCBA bearing pL1 | This study |
MGL2 | MGL1 bearing pL1 | This study |