Skip to main content

Advertisement

Table 1 Plasmids and strains used in this study

From: High production of fatty alcohols in Escherichia coli with fatty acid starvation

Plasmids/strains Relevant characteristic(s) Reference/source
Plasmids
 pMD™ 18-T Vector T-easy vector Takara
 pTrcHisA Expression vector Invitrogen
 pKD46 Bla γβexo temperature conditional pSC101 replicon [8]
 pEASY-cat-sacB T-easy vector with cat-sacB cassette Lab collection
 pL1 pTrcHisA containing the M. aquaeolei FAR gene This study
Strains
 W E. coli K-12 MG1655 Lab collection
 MGKC E. coli K-12 MG1655 ΔtesC This study
 MGKB E. coli K-12 MG1655 ΔtesB This study
 MGKA E. coli K-12 MG1655 ΔtesA This study
 MGKCB E. coli K-12 MG1655 ΔtesC ΔtesB This study
 MGKCBA E. coli K-12 MG1655 ΔtesC ΔtesB ΔtesA This study
 MGL1 E. coli K-12 MG1655 ΔtesC ΔtesB ΔldhA::kan Δpta ΔackA This study
 W/pL1 W bearing pL1 This study
 MGKC/pL1 MGKC bearing pL1 This study
 MGKB/pL1 MGKB bearing pL1 This study
 MGKA/pL1 MGKA bearing pL1 This study
 MGKCB/pL1 MGKCB bearing pL1 This study
 MGKCBA/pL1 MGKCBA bearing pL1 This study
 MGL2 MGL1 bearing pL1 This study